Against and Grey Area Essay

Yet So Colorful To truly understand why gay marriage is a pressing issue, understanding both arguments is a must. The argument across the nation is, are you for or against allowing legally recognized marriage between homosexuals to co-exist with that of heterosexual marriages? Yet should same sex marriage be legalized throughout the entire United States? It is a topic of great disagreement among many people, and is discussed in many presidential debates. Tolerance is a word that should be used in all three of our writings, yet for this neutral approach towards same sex marriage tolerance is a perfect word. A definition of tolerance is: The ability or willingness to tolerate something, in particular the existence of opinions or behavior that one does not necessarily agree with. (New Oxford American Dictionary; New York 2001). In this case, tolerance would refer to being tolerant towards those who are for same sex marriage and those who are against same sex marriage. Tolerance is one of the most basic reasons people have immigrated to the United States. Equality and tolerance are needed, for it is our birthright as Americans. Tolerance is a word that is used in The Constitution, The Declaration of Independence, The Gettysburg Address, and Martin Luther King’s famous speech. As much as I am emotionally supportive of same sex marriage, I do believe that as Americans we must give everyone the freedom to believe what they wish, as long as it is not hurting anyone. Therefore those who disagree with same sex marriage have as much right to disagree with gay marriage as those who are for gay marriage or wish to marry some one of the same sex. Tolerance and equal rights are two major reasons so many immigrants from all over the world selected the United States for their home. These three quotations from famous yet well-respected Americans help to point out values that are important to American society. â€Å"We will only attain freedom if we learn to appreciate what is different, and muster the courage to discover what is fundamentally the same. America’s diversity offers so much richness and opportunity. Take a chance, won’t you? Knock down the fences which divide. – Justice Thurgood Marshall The highest result of education is tolerance. † – Helen Keller, Optimism (1903) â€Å"Laws alone cannot secure freedom of expression; in order that every man present his views without penalty there must be spirit of tolerance in the entire population. † – Albert Einstein So tolerance toward anyone and their beliefs whether you agree or disagree is the correct approach. Homosexuals have the right to express themselves in the same way that heterosexuals express themselves. We all have equality and must be tolerant of each other. Like the vast majority of Americans, I’ve opposed same sex marriage, but I’ve also opposed unjust discrimination against anyone, for racial or religious reasons, or for sexual preference. Americans are a tolerant, generous, and kind people. We all oppose bigotry and disparagement. But the debate over same sex marriage is not a debate over tolerance. It is a debate about the purpose of the institution of marriage and it is a debate about activist judges who make up the law rather than interpret the law. Mitt Romney 12/14/2006 National Review Online In this quote, Mitt Romney argues that Americans recognize marriage as a partnership between man and woman. He states that the creation and development of the next generation is at stake with same sex couples. On the other side of the spectrum gay rights activists argue that marriage should be universal with no boundaries. Gay people (men and men and women and women) should have the same ability to marry just like their heterosexual counterparts men and women. Many gay people claim that not allowing gays to marry is a hate crime. They say it is exactly the same as discriminating against race or religious preference. Thomas Jefferson uses the Declaration of Independence to elaborate on the rights of a government. His idea of equality is closely related to the concept that, because all men are equal, the government’s power is based on â€Å"the consent of the governed,† not by its own arbitrary whims or motives. Today, America is one of the most culturally diverse nations in the world. Given this, the importance of tolerance in the government has great importance at present. The Massachusetts Supreme Court ruled that a ban on gay marriage was unconstitutional, and in the opinion stated that: â€Å"government attorneys failed to identify any constitutionally adequate reason to deny them [gay couples] the right. † This was a very important and groundbreaking ruling. It opened the doors for more states to legalize same sex marriage. It is important for heterosexual people to be tolerant towards homosexuals and their legal right to ask for same sex marriage. It is also important for gay people to respect, be patient and tolerant of heterosexual that do not believe in same sex marriage. Perhaps with tolerance and understanding from both parties more acceptances of each other’s differences will be reached. Change is constant yet does take time. It is scary yet change must happen before we pass judgments. Alex Snyder 9/27/12 C. Dowling Engl. 100 The Wrong Choice, Vote Yes For the Two thousand years of this countries rich history, homosexuality was considered a sin and bad enough to ban, with many state laws prohibiting sodomy. Then in the 2003 Lawrence VS Texas case, The Supreme court ruled in favor of homosexuality and for the laws to be taken away. As the article I read stated, suddenly, homosexuality was not bad enough to prohibit and now, we have to permit it. Homosexuality is bad for the economy and the people of the United States of America and it should be against the law to act in a homosexual manner. My first argument is that a child a gay couple raises may grow up to be shunned by others even in fact alienated by the society they live in. Gay couples raising children cannot give or meet the child’s needs that many heterosexual couples can. Children feel alienated or embarrassed when in public with their homosexual parents. Many homosexual relationships end very badly. Homosexual relationships have a higher break up rate or â€Å"divorce† rate than heterosexual couples. These couples indoctrinate their children from day one on this earth by accepting homosexual relationships as equivalent and the norm. Further more adoption in the homosexual relationship will make the child a trophy, which in effect, validates homosexual relationships. Allowing same sex marriage validates homosexual relationships. My second point is that of taxes, taxes kill me on a day-to-day basis, and I am sure I am not alone. Income taxes will increase in order to make up for the marriage tax benefits given to homosexual couples. As well as paying for social costs resulting from illegitimacy increases. Do you believe Homosexual couples should get the same benefits from raising a child as a man and women raising a child should? Further more social security taxes would increase in order to pay enefits of losing a significant other, such as â€Å"widows† and â€Å"widowers†. Not to mention medical insurance premiums will be at an all time high if gay marriage is allowed because we have to offset the health care costs that go along with homosexual behavior such as (AIDS, hepatitis, Colon Cancer, and other diseases) these homosexual acts will likely be more validated and will likely increase if same sex marriage is approved. Homosexual supporters may say, â€Å"It isn’t hurting anyone† When in theory it is hurting everyone. They could also say something along the lines of â€Å" If I want to behave a certain way, it is my right† when It may be your right, but that does not mean society as a whole is going to endorse and support your choices. Homosexuality will tear this country apart. Hurting our generations to come by indoctrinating our children and our children’s children, but also hurt you and our country as a whole. Alex Snyder 10/7/12 C. Dowling Engl. 100 Gay Marriage: A 21st Century Prejudice Isn’t it good for society to have couples that are in love and are committed to one another regardless of sexual orientation? Furthermore people who love one another create good in there own lives and for people around them. For example I have had the rare opportunity to have two gay couples in my family. My great uncle Bill and his boyfriend uncle Paul were in love and together for over 40 years. They were monogamous and married in their hearts and minds for over 40 years. They taught my siblings, my cousins and I how to sail, cook and love your partner unconditionally through the good times and the bad times. They could never legally be married. The unfortunate consequence of the two never being able to marry occurred when my uncle Bill died this year. Our uncle Paul is unable to support himself because unlike a wife he cannot receive his partner social security benefits and veteran health insurance benefits even though he cared for our uncle Bill for 10 years when he was a quadriplegic. Another example is my step cousin Sylvia and her partner Katherine. They have been together for 20 years. They live in Vermont. They were able to get married. They now have 2 children. Katherine and Sylvia are protected legally and financially through their marriage. They love each other and are committed to being each other’s spouse and being loving parents to their two heterosexual children. They love their children and will support their daughter and son to be the best citizens they can be. Their sexual preference is unimportant. These couples are two examples of how same-sex marriage can be successful. So is necessary to protect the partner if death or illness occurs. For example if a gay person is hospitalized and they are not married to their partner. Their partner is not allowed in the hospital room because they are not family. If their partner dies and they are not married they cannot receive their partners social security benefits but a legal spouse can receive their husband or wife’s benefits. This unequal playing field is sad and emotionally unappealing. With the divorce rate being over 60% and with the suicide rate being so high there should be absolutely no shame or judgment in being with the person you truly love. I might sound like a romanticist yet it may make the world a happier place with everyone being so equal and on the same ground, giving way for more tolerance and love to be expressed.

Employment and Family Essay

This paper is about Family Related Issued: Family and Medical Act, in studying the case where it matters that a parent literally had nothing to do with a biological child in order for the child to take advantage of the Family and Medical Act (FMLA) to care for that parent. The motive in this paper is to determine the size of the business can have any effect on whether Toney is eligible for family leave under the FMLA. I will discuss whether Herman can or cannot imply that if Tony takes a leave of absence under the FMLA, he may not have a job when he comes back. In this paper I will give describe who is covered by the Family and Medical Leave Act (FMLA) of 1993? In this case I will explain the extent to which an employer can make his or her own determination as to the eligibility of an employer under the Family and Medical Leave Act. Explain if it matters that a parent literally had nothing to do with a biological child in order for the child to take advantage of the Family and Medical Leave Act (FMLA) to care for that parent. The Family and Medical Act is a law endorsed by the United States that needs big companies top management team to offer its employees, the coverage to (FMLA) for their job compensations due to a short term or long term serious health illness that effect the employee to be off from work because they need to assist a family member, spouse pregnancy, or care of children. In the case it did not matter if Tony was his biological son or not. In the Family and Medical Leave Act there is a limit on the amount of sick and vacation time that can be used and it is apparently stated in this law. Also, if there is enough time they are allowed to by law. With a lot of companies they now used PTO days that include vacation and medical days at work that employees can use after six months or one year depending on the business. In was not specified in the video how long Tony had been employed with the company, as the request was for three weeks off. Under normal circumstances the business would ask foe a written request for the leave of absence or medial leave on the FMLA form. And the company would also like to have at least a two week notice in most causes so the employee would have time to get a substitute while Tony is out on leave. Explain whether the size of the business can have any effect on whether Tony is eligible for the family leave under the FMLA. Herman thinking and his thoughts was a concerned for the company, but the business size does not matter. Herman is utilizing Tony as a business prerequisite from medically taking care of his father that is in need of his assistance. This companies has more that enough employee that could cover Tony while on leave, though Herman shows concern about the sales of automobiles being sold due Tony absence. Herman main issues of Tony taking time off is because of the affect it will have on their business. Even more this makes Tony eligible for the FMLA during this time. (Halbert, Ingulli 2010) Explain whether Herman can or cannot imply that if Tony takes a leave of absence under the FMLA, he may not have a job when he returns. Herman cannot imply if Tony takes a leave under the FMLA, but he must make Tony aware that there is not guarantee that he will have a job when he is able to come back to work. This kind of action or treatment is not fair at all and the FMLA Act protects the employees if they are eligible to take a leave to assist their family of serious illness, to take care of newborn, or accommodate other family responsibilities. Because Tony is a valuable employee, one of the companies top auto salesman by word of mouth as number one marketing strategy. Herman has a great concerned, that he may not have another strong salesman that could fulfill the service like Tony provided for the company and to the customers (2010). Describe who is covered by the Family and Medical Act (FMLA) of 1993. The Family and Medical Act of 1993 were put into place to promote the stability and economic security of families as well as the nation’s interest in preserving the integrity of families. The Family and Medical Act applies to any employer in the private sector who employers 50 or more employees each working at least 20 calendar seeks in the current or preceding calendar year. This law covers all state, local governments, and local education agencies. Title II of FMLA covers most federal employees, who are subject to regulations issued by the Office of Personnel Management. Also, the employer can not use the practice if forcing employees to behave in an involuntary manner such as threats or intimidation of their jobs should they chose to take a leave under the FMLA. (Halbert, Ingulli 2010). Explain the extent to which an employer can make his or her own determination as to the eligibility of an employee under the Family and Medical Leave Act. An employer determines the eligibility of the employees based on the annual worked hours by the employee. Human Resource also request that a written FMLA form is completed with end two weeks of the request leave. Should an employee have not been notified within a 30 day the employer can make a decision of eligibility, causing a deferral of the FMLA? In addition, an employer is providing medical certification which would prove the leave would clarify eligibility for leave. If there is reason to believe by the employee that the employer does not plan to return to work at the end of the leave the employee has the right to terminate the FMLA request. Employer also has the right to terminate the FMLA as well if there falsified information given by the employer. Halbert, Ingulli 2010). The Family and Medical Leave Act is a benefit for companies, employees and families in the place of work to allow ones job functions, and personal responsibilities to be taken into consideration. During the time of leave the employees receive an income and security doing the time off. Other benefits to employees is receiving aide from relatives in the time of need, and the organization have an advantage from yearly d ecrease in staff earnings, which will lower the preparation and employment costs, and improves the performances of the employees. There have been thousands of employees that have used the Family and Medical Leave Act (FMLA) and business programs of essential to developing effective impact reporting that is positive or concentrating on improving the performance of their employees, and allowing the employees to know that there are limitation in the law for exclusion of more than thirty percent of employees that do not meet the criteria for the leave, which would prevent the employees who meets the criteria from going on any leave due to there is no extra funding or budget to cover the cost. Halbert, Ingulli 2010). Conclusion In conclusion of the Family and Medical Leave Act the matter that a parent literally had nothing to do with a biological child in order for the child to take advantage of the law was enacted by the United States and requires large business to provide their employees with this coverage to protect the employment for family related issues such as a serious health illness or care of children. Secondly, this law is also effective with companies that employers that have at least 50 or more employees on staff. Thirdly, Herman cannot imply if Tony takes a leave under the FMLA, and in this law he must make Tony aware that there is not guarantee that he will have a job when he is able to come back to work. Fourth, in this case in the year 1993 the FMLA was presented to employers for employees that are eligible for the leave of absence and met the criteria required for the companies. And last we have explain the extent to which an employer can make his or her own determination as to the eligibility of an employee under the Family and Medical Leave Act when the hours worked in a year time, written notice submitted to human resource has been submitted in a timely manner and medical certification has been documented will allow the employee to have the time off on leave.

Admissability of Independent Expert Reports Essay

Admissability of Independent Expert Reports - Essay Example This paper discusses the admissibility of an independent expert report in a court of law or other judicial forums. It identifies and explains concerns or issues about the characteristics that a witness must possess in order for a court Royal Commission or other duplicate resolution forum to accept him or her as an expert. It also discusses the factors that mark an expert report as either admissible or inadmissible as evidence, the factors that make a witness and expert evidence credible and the factors that limit the credibility of a witness and/or expert evidence tendered. The paper meets its objectives by citing major cases and case laws, literature, judicial surveys among others. An expert is one who concerning any question, is knowledgeable or experienced on that question in such a way that in evidence; his or her view would be admissible on that question. An expert witness therefore is an expert who in proceedings or proposed proceedings, gives opinion evidence or provides a report with regard to his or her opinion to act as evidence. If technical, scientific or other special line of knowledge will help the judge to determine a truth in issue or understand the evidence, a witness who qualifies as an expert through education, knowledge, training, experience or skill may give evidence thereto either in form of an opinion or otherwise (RichmondTrial Lawyer.com 2009). It is hackneyed law that witnesses who possess a relevant degree of expertise have the competency to give evidence on the factual matters lying within their specialty to offer assistance or guidance to the judges in their ruling of the factual issues in dispute. Indeed, the adduction of expert evidence is sometimes mandatory in criminal proceedings. The courts recognize experts per se either for the reason that they possess relevant qualifications professionally or due to their relevant practical experience, or

Request for proposal Essay Example | Topics and Well Written Essays - 1500 words

Request for proposal - Essay Example The faculty of engineering for time now in Cleveland University is a tool that has for seen many students come out to emerge as challenging and experts in these fields. Computer and electrical engineering has imposed more technological heighten and with it several of the fields of study are improving in machine orientation. Fields of business, medical, survey, planning, architecture among others relies on electronic competence to accomplish and abridge their work. Still today many engineering students are Specific aims Computer and electrical engineering are faculties that are proving to be the backbone of the technological innovations. Still there are many challenges behind the teaching of the faculty courses in offering what is really required of the program to have. Propose for the research in Cleveland university is to have: 1. Equipped laboratory with modern machined and tools to help in teaching. More of the laboratory to be added are Digital system research laboratory Digital communication research laboratory Software engineering research laboratory 2. ... Background and significance of research problem Engineering is defined as design application of theoretical principles and engineering experience to the practical solution to technical problems. Engineering in Cleveland state university is a faculty that has been prosperous in both the theory teaching and practical and this has made is possible to offer up to doctorate level in academics. The program includes required courses and an integration selection courses in the student field of interest which are communication system, computer systems, control and power and power systems. Modeling the engineering field will help the student come out with a lot of experience. Since the university has an internal workshop for students and also a research for masters and PhD students, there have been more setbacks in machine orientation and layout but with the addition of this then definitely there will be bulk in terms of equipping students. The aim and the objective of the university is to mou ld competent engineers in their respective faculty. Iam concerned with this research because it is aiming at educating students and making them ready to face the real world with confidence. Student develop their interest and motivational skills when they are shown the way and how things are done and this is a better way of ensuring that students are thought how to think but not what to think. It's obvious that most have a sense of community, but the question then becomes how to help them transfer some of that sense of community to what we consider to be "learning". Pardon the quotes around those, but I'm appropriate more persuaded on our curriculum does not have as outsized effect on what the students are learning as we suppose. Our school aspiration for the year is â€Å"Focus

Thea Essay Example | Topics and Well Written Essays - 1250 words

Thea - Essay Example During this era, Hollywood or cinema was becoming an imperative part in the development of the fashion industry. Cinema became the mode of shifting attention from the sorrowful scenario generated due to war. Hollywood actresses played crucial roles in determining the fashion statements of the year. Uche Okonkwo in the renowned book; â€Å"Luxury Fashion Branding: Trends, Tactics, Techniques† mentioned, â€Å"This led to the increase of the style and fashion influences of Hollywood actresses like Marilyn Monroe, Liza Taylor, Audrey Hepburn and Brigitte Bardot† (Okonkwo 30). THESIS STATEMENT This essay intends to focus on the influence the costumes of the movies in 1940s had on the general fashion of the era. Moreover, keeping in view the two prominent actresses who were extremely popular in that generation in Hollywood, Audrey Hepburn and Katherine Hepburn, the essay focuses on the stylistics and fashion statement of these two divas on the society as a whole. FASHION OF HOLLYWOOD IN 1940s To understand the fashion and style of Hollywood films during 1940s, it is essential to study the nature of film produced during the era and the characteristics and features of those films particularly. Film Noir is a product of these decades, but 1940s and 50s observed the increase of Hollywood musicals as well. The decade viewed a mix feeling of emotion and maturity in the films. With the disappearing of the war generated distress among people, it was a suitable time for the people to celebrate a new beginning and reunite. Depressing activities of war time started eradicating slowly during this decade. In its place, action, adventure and fun began to take priority for the subject of the movies. Taking the opportunity, Hollywood started focusing on entertainment, making it a component for profitable film making. This kind of fun, adventure and family dramas did involve a considerable amount of scope for fashion and decorative settings. People were significantly m otivated towards following the decoration appearing on the film sets and the hair style, shoes and bags along with jewelleries worn or used by their dream man or woman on the silver screen. ROLE OF ACTRESSES IN DETERMINING THE FASHION STATEMENT OF 1940S: A CLOSE STUDY OF STYLISTICS OF AUDREY HEPBURN AND KATHERINE HEPBURN The movies of 1940s did give birth to a number of new actors and actresses in Hollywood films. The emergence of such new talents brought with it a combination of new tastes and new faces which positively altered the meaning of cinema. It also expanded cinema totally from a very conventional form. The spectacular aspects depicted in the films started to get apparent through the costumes and fashion statements of the heroines. It would be unfair to take the name of one of the timeless beauties of Hollywood when fashion statement and its depiction in cinema become the topic for discussion. Audrey Hepburn made a stunning performance in the film ‘Roman Holiday†™ in the year 1953. As regards to her fashion statement, it is quite necessary to mention that Audrey Hepburn has made her space in the International Best Dressed List Hall of Fame. The appeal and style with which she carried all her apparel added an extra attraction to them. Actually, 1940s witnessed the huge addition of women into the film industry. Thus, the pioneers like Hepburn had to take the extra care of maintaining the dignity and respect of women appearing on screen as icons of independent American

Undecided Assignment Example | Topics and Well Written Essays - 1000 words - 1

Undecided - Assignment Example The project personnel was present to answer the questions and concerns raised by the members of the public. The scope of the proposed project would widen the existing six-lane road facility to an eight-lane road highway and in addition, extend the shoulders of the road by reconstructing the existing two-lane frontage roads and upgrade to meet the current standards. It was proposed that by improving the infrastructure, the economic growth of the city will increase because of reduced traffic delays, improvement of safety in the city and it shall facilitate evacuations during an emergency. Due to the expansion project, an additional 11-acre stretch of land was required to complete the project successfully. It meant that several businesses shall be displaced by the improvement; however, the personnel manager present provided procedures, programs, benefits and other information on land acquisition. The environmental document and another drawing showing the proposed project was displayed t o the public hearing as well as other tentative schedules of how the program shall be executed. At 1400HRS, the expected speakers and the public had arrived and taken the various seats and position. A short prayer was offered and the was started by the city clerk who took us through the norms of the meeting. The first part of the discussion involved free intermingle among the public and the project developers, this was very interesting since it gave the public to have a close interaction with the project managers. It further enhanced rapport and close understanding of the project since several people were able to ask questions, which they may not have a chance to ask during the actual presentation. After a one hour of interactive mingle and discussion, everybody took back their seats for the full technical presentation of the project. The personnel project manager was given a chance to take us through the whole project, stating its merits and it is going to affect the citizen.

The role of state fusion centers in preventing terrorist acts Term Paper

The role of state fusion centers in preventing terrorist acts - Term Paper Example Furthermore, most of the security agencies were not on a very high alert for any foreign attacks on the United States because such threats were considered nonexistent. Instead, security agencies concentrated on the potential from within and this was a huge flaw on their part. The American military had bases all over the world in allied countries and these had been put in place to protect American interests in these respective regions. The military had not seen much active service since the 1991 Gulf war and its presence in allied countries functioned as a deterrent to those who would wish to harm American interests. All these, however, changed after the attacks and the need for cooperation between the different security agencies in the country was realized. The origin of state fusion centers creation provide a means through which information can be gathered and shared between different law enforcement agencies, ensuring that the terrorist threat to the United States is recognized ear ly and dealt with swiftly. State fusion centers are facilities that have come to allow investigators to pool data from local, state and federal sources to ensure that the terrorist threat to the country is averted. ... The system was created to ensure that investigators had all the information they needed in order to perform their tasks effectively, and it allows local investigators to access the federal Homeland Secure Data Network. The system has been designed to provide individuals in security agencies access to classified reports from federal agencies like the Department of Homeland Security and the Federal Bureau of Investigation. This has made up for the previous lack of information sharing between security agencies and has created a situation where it has become easier for terrorist threats to the nation to be detected and whenever there has been any danger, it has been swiftly countered so that the nation has remained secure (Desai, 2005). State fusion centers have become indispensable in the fight against terrorism on American soil and this has justified their continued existence of this system. The state fusion centers help security agencies to look for connections among potential terrori st suspects so that they can find more information concerning them and the danger that they may pose to the people of America. They do this by looking through hundreds of law enforcement sources around the region and these carry a large amount of information concerning individual suspects and their associates. The information that is often investigated ranges from traffic violations, to jail records and criminal histories. Furthermore, this also includes information concerning public records such as property records. As the investigation widens, and investigators identify suspects, the system can help lead authorities to known associated of the suspects who may be involved in further terrorist plots against the country or its interests elsewhere (Ratcliffe and Guidetti, 2008).

Faisal - finance Essay Example | Topics and Well Written Essays - 3250 words

Faisal - finance - Essay Example Therefore, the most appropriate financial theory that is applicable to the company is the trade-off theory. The company is trying to balance cost and benefits associated with both equity and debt. Source of capital in the company is equity, bonds, bank loans and finance leases among other financial liabilities. Each of the above sources of capital has both advantages and disadvantages. It is possible to compute value of the company by calculating weighted average cost of capital (WACC) and shareholder value. WACC refers to an average rate of interest at which a company is expected to pay all its providers of capital. The seven drivers of shareholder value are growth in sales, operating profit margins, tax rate, working capital growth, fixed capital growth, cost of capital and the period of competitive advantage. The total value as estimated by the shareholder value drivers of the company was â‚ ¬16603.7 million in 2019. When the shareholders drivers are increased by 5 percent the company value of the company will increase to â‚ ¬21,443.61 million. The disadvantages of shareholder value analysis include difficulty in computations, difficulty in implementation, failure to include social needs and possibility of making errors in calculation of shareholder value analysis. ... Capital structure According to Taylor and Sansone (2007), capital structure is determined by the composition of target collateral pool, investment flexibility, condition existing in the market for the collateral, weighted average cost debt capital and the desired ratings of the bonds in the capital structure. Capital structure can be analysed by comparing company’s debts and equity used to purchase company’s assets. The capital structure of TUI AG is defined by shareholders funds, various categories of corporate bonds, finance lease as well as bank loans. To understand the theory behind the capital structure, it is important to explain the various sources of finance and the impact on the company. Leverage ratio of TUI AG is 62 percent (figure 3: in the appendixes). This indicates that 62 percent of the total assets were funded through debt. Table 3- Sources and Amount of capital of TUI AG for 2010 Source of capital Amount in million â‚ ¬ Equity 2434.2 Bonds 3038.3 Ba nk loans 1001.5 Finance leases and other financial liabilities 472.1 Source: TUI AG 20104; CIA 2011; TUI AG (20106) Finance theory There are a number of theories that have been used to discuss capital structure in companies. There are Modigliani-Miller theorem of capital structure (capital structure irrelevance), the pecking Order Theory and Trade-off theory of Capital Structure. Modigliani-Miller theorem of capital structure states that in absence of bankruptcy, transaction costs and taxes in an efficient market and asymmetric information, the value of the company is not affected by how it is financed. This theory is not applicable at this time because there are taxes. There are tax benefits because the value of the firm is decided after tax has been deducted. Table 1;

Discuss what you believe to be the purpose(s) of education today Essay - 1

Discuss what you believe to be the purpose(s) of education today - Essay Example â€Å"From various parts† is depicted by the fact that there are students and professors for each and every department of knowledge. In its rudimental and simple form, it’s a school of knowledge of each kind, consisting of learners and teachers from every quarter. A university in its sense seems to be a place for the circulation and communication of thoughts, through means of personal intercourse, over a wide range of country. A university contemplates our nature’s necessity and is one specimen within a particular medium, from many which could have been adduced in others, with a provision for that necessity. In a broad sense of word, mutual education, is among the incessant and great occupations of human society, that is carried on partially with set purpose and partially not. One generation forms the other; the existing generation is acts and reacts upon itself within the people of the individual members. Books, in this process, are one special instrument. It’s emphatically so and true in this age. Putting into consideration the prodigious press powers, and the way they are developed during this time in the never intermitting issue of tracts, tracts, periodicals, works in series, light literature and pamphlets; we have to allow that there was never a time that fairer dispensing every means of instruction and information. Spayde Jon (2000), views street education and college education as the different aspects of education. The question however is that among the two educational aspects, which one is more necessary and relevant for survival in the world today. Maybe education cannot provide the necessary tools for survival in the real world. As much as we aren’t wasting time in institutions; the real world usually is a bigger school as compared to the other schools that we attend. The definition of school in the 21st century, unfortunately, is technology for instance. Spayde Jon (2000) argues that for our

The Political Philosophy of the Constitution Essay Example for Free

The Political Philosophy of the Constitution Essay From my own perspective I feel that the major ideas of the political philosophy of the constitution are to mandate non-partisan redistricting for elections to enhance electoral competition. For example: To reduce the role that legislative politics might play, five states (Arizona, Hawaii, Idaho, New Jersey and Washington), carry out congressional redistricting by an independent or bipartisan commission. Two states, Iowa and Maine, give independent bodies authority to propose redistricting plans, but preserve the role of legislatures to approve them. Seven states have only a single representative for the entire state because of their low populations; these are Alaska, Delaware, Montana, North Dakota, South Dakota, Vermont and Wyoming. Redistricting is also necessary within school districts, where attendance zones have grown (or occasionally shrunk) disproportionately to the occupancy capacity of each public school in the system. This always occurs when a new school is built or one is closed, but may also occur due to other shifts in population. These districts are necessary not only to balance enrollment, but also to coordinate school bus routes. Separate maps are usually kept for each level: elementary school, middle school, and high school, for example. This is not an inherently political process, however parents can become very upset when their children are moved from a school they like (or to one they dont), and occasionally elected school boards have been forced to change plans after protests. 2nd Eliminate lifetime tenure for federal judges in favor of non-renewable 15 years term for all federal judges. Judges and certain members of some senates or upper chambers (senator for life) most commonly have life tenure. The primary goal of life tenure is to insulate the officeholder from external pressures. And last a Political meeting which we know as a constitutional convention is a gathering for the purpose of writing a new constitution or revising an existing constitution. A general constitutional convention is called to create the first constitution of a political unit or to entirely replace an existing constitution. An unlimited constitutional convention is called to revise an existing constitution to the extent that it deems to be proper, whereas a limited constitutional convention is restricted to revising only the areas of the current constitution named in the conventions call, the legal mandate establishing the convention. Constitutional conventions have also been used by constituent states of federations such as the individual states of the United States to create, replace, or revise their own Constitutions though several states have never held a national constitutional convention for the purpose of proposing amendments, the 21st Amendment to the US Constitution was ratified not by the state legislatures, but by state level conventions after it was passed by Congress, as described as an alternate method of ratification in Article V of the US Constitution. Furthermore, The systematic elaboration of the consequences for politics of suggested resolutions of philosophical dilemmas (or of the intractability of those dilemmas). The greatest works of political philosophy try to present those consequences in relation to fundamental cosmological, ontological, and epistemological issues. They articulate a view of human nature which links the cosmological with the political. On a less grand scale, political philosophy explores the political implications of particular disputes, for example about the nature of the self (see communitarianism; freedom; liberalism; and autonomy), or about the notion of moral responsibility (see punishment). There is obviously a close connection between political philosophy and moral philosophy, because both involve exploring the nature of judgments we make about our values; consequently, when it was thought on epistemological grounds that it was not the place of philosophy to explore these normative matters, political philosophy was declared to be dead. Contemporary political philosophy flourishes because the epistemological argument once thought fatal to it has Do we need a Constitution. Yes! we need a Constitution because The founding principles behind the Constitution, were values created by men enduring the stress and pressure of creating a new union aside from the rule of Great Britain. Much debate and thought about human values and the rights of a free people were instituted in the writing of this famous document that has endured in the United States for nearly 232 years. Although there have been a variety of amendments, the basic content has been respected and used to govern in the USA throughout history up to the present. The majority of citizens believe in this document to govern our country as its tradition is valued still by many. Just like the Bible is a guide to many religious denominations, the constitution is a guide to the structure, laws and behaviors influencing the culture in America. In conclusion I hope that my ideology on major ideas of the political philosophy of the constitution are true and not of opinionated facts. I obtained my REFERENCES from: Hampton, Jean (1997). Political philosophy. p. xiii. ISBN 0813308586. http://books. google. com/books? id=-. Political philosophy is about political societies. ^ Sahakian, Mabel Lewis (1993). Ideas of the great philosophers. Barnes Noble Publishing. p. 59. ISBN 1566192712. Kraut, Richard (2002). Aristotle: political philosophy. Oxford University Press. p. 3. ISBN 0198782001. (Cicero: a study in the origins of republican philosophy. .http://books. google. com/books. By the ninth and tenth centuries ^ Gellner, Ernest (1992). Plough, Sword, and Book. University of Chicago Press. p. 239. ISBN 0226287027. Natural Law and Calvinist Political Theory. Trafford Publishing. p. 19. ISBN 1412007382.

Plants in Production of Recombinant Antibodies

Plants in Production of Recombinant Antibodies Shirin Bagherihanaei A discussion of the techniques, advantages and disadvantages of the use of plants in production of recombinant antibodies for research and therapeutic use with named examples. Introduction   Ã‚   Application of plant for medical proposes goes back to thousand years ago. Our ancestors use plants and extract its beneficial substance to cure different illnesses and relief pain. This idea is continued with us and today we can manipulate the genetic information of plants to make them suitable for the production of recombinant protein and biopharmaceutical medicinal purposes [1]. Since the first generation of recombinant protein from tobacco cell culture, a variety of pharmaceutical products have been introduced such as vaccines, hormones, antibody, growth factors, and cytokines [1,4,9]. However, AB is the most common recombinant protein which is generated by plants and it has been called plantibody. Nowadays the development and the use of transgenic plants for production of recombinant ABs is an attractive subject among scientists because plants are easy to work with and also the cost of the production is considerably low. It is also beneficial because of the large-scale productio n [1]. Production of high quality recombinant protein for research and therapeutic purposes from mammalian are quite expensive, therefore the idea of producing recombinant protein in transgenic animals and plants has formed recently [1]. In this essay, I try to summarise and highlight some of the most cutting-edge techniques in the use of transgenic plants for production of recombinant protein and antibody. I also discuss their advantages and disadvantages with the utilization of plants to produce antibody. Plantibody Plantibody made up of two words: plant and antibody. It means plantibody is an AB that is generated from plants. Antibody is a glycoprotein which mainly made by plasma cells and used by the immune system to neutralize any kind of foreign molecules inside the body such as bacteria and virus. Plantibody has this property to recognize and bind to its specific antigen. It can be generated from tobacco, rice cells culture, Lemna minor (duckweed), Arabidopsis thaliana seeds, Medicago Sativa (alfalfa), lettuce and maize [2] but tobacco is the most common source due to its high leaf biomass yield [3]. According to a report, recombinant protein level in tobacco stem is the same as this level in its leaf. That means to produce recombinant therapeutic protein, the whole plant biomass can be used [3]. Another advantage of tobacco is that it is not edible and this aspect of tobacco reduce biosafety concern but it contains toxic alkaloid and the plant should be purified from the toxic chemicals [3]. As tobacco is not an edible source, regulatory issue for production of recombinant protein is less controversial than food crops such as rice, soy bean and corn. Chinese cabbage has the highest amount of soluble protein among plants. Production Techniques Production of the recombinant protein includes utilizing the whole plant or plant cell culture in vitro [9]. The disadvantages of using the whole plant for production of recombinant AB are: time-consuming generation of transgenic plants, the risk of contamination with fertiliser, unstable quality and yield of the products, applying good manufacturing practice (GMP) to the whole-plant production pipeline [9]. Plant cell suspension culture has the benefits of both mammalian cell culture and whole plants. Undifferentiated plant calli can be developed under a proper condition in the liquid media environment and produce cell suspension culture. Plant cell culture can generate proteins which are more similar to human generated proteins. They can also grow rapidly in a simple media same as bacteria. Plants are eukaryote so they have fairly similar post-transitional modifications such as glycosylation that happen in human cells [9]. Glycosylation is an enzymatic process that glycan adds to o rganic molecules such as lipids and proteins. Correct pattern of protein folding is also essential for recombinant protein to function [1]. It is interesting to note that plant suspension cell culture lack fully functional plasmodesmata, therefore, systemic post-transcriptional gene silencing (PTGS) may be reduced because PTGS is transmitted through plasmodesmata and the vascular system [9]. Generally, three different methods are applied in the production of recombinant AB in plants: Agroinfiltration with recombinant agrobacteria, particle bombardment technology and Infection with modified viral vector [8,14]. The general technique for the production of genetically modified plants is agrobacterium-mediated transformation [2]. Agrobacterium Tumefaciens is a gram negative bacteria which is the cause of crown gall disease in plants [14]. These bacteria live in soil and attract to the plants with wounded parts. However, scientists use this bacteria as a tool for research and therapeutic purposes by introducing the gene with desired properties into the plant cells in plant genetic engineering. The gene of interest can be inserted into Ti plasmid (tumor inducing) then injected into the plants as a host. Plant cell divide out of control and the gene of interest proliferate as well [14]. There is a selectable marker on the T-DNA which is transferred into the host cells therefore it is possible to control if the gene is transferred successfully or not [2]. There are two transformation strategies for generation of recombinant antibody, Stable and transient expression. Stable expression is the stably insertion of cDNA encoding both heavy and light chains of AB into the genome of plants. The gene can be introduced into the chloroplast genome to produce chloroplast transgenic plants which can generate AB with correct folding and disulfide bonds. Some example of the transient expression is agroinfiltration and recombinant plant viruses for the production of antibody [3]. Agroinfiltration system has been used to produce multi-antennary N-glycan that mostly seen in mammalian derived glycoproteins [3]. Transient expressionmethod is fast and convenient for the production of recombinant antibody without generation of transgenic plant. The generation of transient expression is the precondition to stable transformation because it can test expression vectors and protein stability and also it is able to recognize any problem that may have happened [ 8]. Transient expression is better for low scale yield protein production yet transgenic plant are better approach for high yield production and also gives a better expression levels [7]. An important point to note is if the expression is targeted to the endoplasmic reticulum (ER), this results in higher yield [7]. Another approach for the insertion the gene of interest into the plant tissue is particle bombardment technology. The main idea of this technique is some microscopic golden bullet or tungsten bullet covered by the gene of interest. These particles are fired into the plant leaf. This technique used for all type of plants. The golden bullet preferably used because the tungsten bullets have the risk of toxicity for the plants. Then the bullet is placed at the end of plastic bullets and shoot with blasts of air or helium. There is a plastic mesh work shop on the way of the bullets which guide the bullet to move forward. An alternative technique used for this approach which can accelerate the beads with strong electrical discharge which results in a controlled penetration of beads into the plant tissue. After penetration of the DNA dissolved into the cytoplasm of the leaf, the gene of interest can recombine with the chromosome of a plant. Finally, the leaf is transferred to media and let it grow and regenerated using tissue culture [8,11]. This technique does not use a lot due to its high cost and also as this method is physical so the insertion of the gene which is performed by gene gun may cause damage to plant without transferring the genetic material inside the plant and dose not give the precise or desirable results [14]. Production of ab transgenic plants can be generated by viral vectors. However low infectivity with this vectors needs to be considered as an obstacle [2]. One of the disadvantages of viral plant system is the injection of vector to leaf or stem every time which can result in gene mutation during replication of the virus. But we dont face this problem in transgenic stable expression. Therefore, it is extremely important to choose the proper protocol for gene ex pression [3]. Advantages and Disadvantages Plants paly an important role as a bioreactor for production of recombinant protein. Basically, the common systems use for the production of recombinant proteins is the manipulation of mammalian cells, bacterial systems, yeast and etc. However, recently due to some negative aspects of these systems many scientists prefer to work and study plant sources which have those benefits that they are looking for. There are several important benefits with the production of recombinant AB from plants. Firstly, is the large scale of production from cheap raw materials and the reduction of costs in comparison with other techniques of recombinant AB production such as yeast, mammalian and etc. [3,5]. Another advantage of using plants for production of AB is the flexibility of working with plants as it can be used both in vivo and in vitro [3]. In addition, introducing new transgenic plants is possible by sexual crosses and they are quite easy to work with. There is a very low risk of contamination by mammalian viruses when AB is generated from plants [5]. Another advantage is correct folding and assembly of produced AB for both single stranded peptides and multimeric protein with full size. Recombinant protein which generated from edible sources does not require purification. In terms of storage the enzymes which are produced by plants can be formulated to the seeds, so under the suitable condition they can be stored for long period of time and it is also possible to transport them to different locations easily. Plantibody have both avidity and affinity towards its specific antigen and its characteristics maintain the same after purification [1]. Although plants have lots of benefits but it is not 100% perfect source for production of antibody [3]. The most important disadvantage is the fact that Plant N-glycosylation is different from human and mammalian glycosylation. Another negative point is that plants has shown discrete yields due to low gene expression level [7]. There is also the problem with causing allergic and immunogenic reactions in humans, which is because of the difference in glycosylation pattern in humans and plant [7]. Moreover, there are some concerns regarding the activity of proteolytic degradation, which might influence fully assembled IgG that is secreted in the culture media [9]. Production of mycotoxin by impurities, limitation which caused by the environmental condition, and the possibilities of herbicides presence in the product are some other negative aspect of transgenic plants [1]. The controversy about plantibody generation is the presence of gene segments or marker segments in the produced drug and its effect on human body and the probability of allergic reaction to plant glycoprotein [1]. Although there are some disadvantages with the use of transgenic animals such as the risk of contamination of protein with animal viruses and also it takes a long time to produce recombinant protein from transgenic animals but, many biotechnologists prefer to produce AB from mammalian cell lines because the final ABs have a correct glycosylation pattern and protein folding [1]. Plant Antibody Application The extracted AB from plants can be used for many different purposes such as vaccine production, clinical diagnosis protein, pharmaceutical and industrial proteins, biopolymer, biodiesel, food industry, tools for research, and diagnosis tool for chromatography and other immunoassays [1]. The application of AB in research is extremely wide, because of their transferability with the metabolic process in organism [1]. Protein pharmaceutical products are one of the most expensive and important products that human has managed to synthesis them in ways other than natural methods. In recent years, mAB has had an important role in the diagnosis and treatment of cancer-related research [3,12]. Each mAB influence cancer cells in 3 ways: it can signal to the immune system to kill cancerous cells, it can prevent the division of cancer cells or deliver drug to these cells [3]. mAB can attack tumour cells by complement system in cytotoxic reactions through complement system. They bound to the tumor cells which prevent tumor growth and finally result in apoptosis [3]. The ability of AB to prevent the pathogens and tumor cells is due to the affinity of the variable binding sites. This affinity of AB could have enhanced by modifying glycon structure and glycosylation patterns [3]. As we see mAB have many positive aspects for prevention of cancer but their application is not common which is duo to the risk of contamination with human pathogens, high cost and proliferation inability. However, these problems have been eliminated by the production of mAB from other bio-organism like bacteria, yeasts, insects, and plants [3]. The monoclonal AB expressed in plants by tobacco mosaic viru s vectors [3]. Nimotozomab is a humanized anti-epidermal growth factor receptor recombinant AB which is produced in animal cell culture. This AB is used for treatment of different carcinoma cells. It seems that a mutation in the N297 position in the IgG1 FC region of this AB and apply it in a transgenic plant which result in producing a form of nomotozomab that is similar to mammalian-cell-produced AB. It also has the property to block the EGFR interaction and have antitumor effects [5,7]. Nicotiana tabacum were transformed by A.tumefaction-mediated gene transfer method. In order to infect the plant cells, recombinant pDEGF-R Agbacterium bearing the binary vector was applied [5,7]. According to experiments the mAB which was generated in plants was as effective as the one which was generated in mammalian (nude mice). In another experiment marine, mAB could prevent Brest cancer cell growth and mAB was generated from transgenic tobacco plant which had the same function as the murine mAB. Therefore, pl ants such as tobacco can produce two different mAB which can target two different types of cancer cells [3]. The most frequently chosen host cell lines used for recombinant protein expression are Tobacco BY-2 (Bright yellow-2) and NT-1 (Nicotiana tabacum-1) cells [9]. Generally, IgA, IgG and IgM are generated from plants. IgA and IgM have the potential for commercial production. They attach antigens in the first line defence at gastrointestinal mucosal surface, tears, saliva and milk [14]. IgG and IgA have been introduced in Nicotiana, Arabidopsis. Plantibody have a high level of safety which rise the interest for production of mAB from plant Examples include the Guys 13 IgG1 (Fischer et al., 1999b; Sharp and Doran, 2001a, 2001b), a human mAB against hepatitis B virus surface antigen (HBsAg) (Yano et al., 2004), a human anti-rabies virus mAB (Girard et al., 2006), and most recently a human anti-HIV mAB (Holland et al., 2010) all of which have been reported to be expressed in tobacco cell suspension cultures [9]. Lots of effort have been done for production of these ABs in large scale but none of them sell in the market due to the high cost. Nonetheless, two plantibody is used in clinical CAROX which was expressed in transgenic tobacco that takes part in the prevention of tooth decay and the second one have an effect against non-Hodgkin-lymphoma(NHL) [2]. The following table demonstrates some IgA plantibodies which are generated in recent research. Plantibody Source Target Plantibody Characteristic sIgA/G Transgenic Tobacco Plant S. Mutans Prevention of tooth decay Human IgA Maize Herpes Simplex Virus and saga 1 antigen Herpes disease and sperm agglutination Coccidia specific chicken IgA Nocotiana Benthamiana Eimeria Acervulina Against the coccidiosis Virus-specific IgA Tomato and Nocotiana Benthamiana Rota Virus Development for passive immunisation against Diarrhoeal disease Chimeric Enterotoxigenic Bacteria-Specific IgA (VHH-IgA) Arabidopsis Thaliana seeds Enterotoxigenic Escherichia Coli (ETEC) Passive Mucosal Immunisation Against Enteric Infections Chimeric Toxic-Specific IgA (Hybrid IgG/IgA) A. Thaliana Shiga Toxin From ETEC Against Haemorrhagic colitis and Hemolytic-uremic Syndrome Monomeric IgA1 K! Variants (Infliximab, Adalimumab, Ustekinumab) N. Benthamiana Against Autoimmune disease 2G12 sIgA N. Benthamiana Human Immunodeficiency Virus Anti-HIV Human This table shows IgA plantibodies, their sources, targets and characteristics. Conclusion and future perspectives Although there are problems with the generation of plantibody from mammalian cells, but they are the most common source for production of mABs. This is due to the correct folding and similar glycosylation patterns to human, complex type N-glycosyl, moieties and the presence of polypeptides with disulfide bonds. Using recombinant antibody fragment in research therapeutic purposes, biotechnology and pharmaceutical science is increasing because of the intrinsic properties of the components such as the ability to penetrate better and detect antigen with higher affinity, small size and easy production compared to AB full size [6,13]. More powerful tissue or inducible promoters, enhancement of transcript stability, translational improvement with cutting edge sequences or strategies and transgenic chloroplast system are some ways which are studied in order to raise the AB expression level in plants in the future [8]. Drug production seems to be one of the promising field in terms of commerc ial development in biotechnology [1]. In total, we can see a promising future for the production of drugs, vaccine, recombinant protein and biopharmaceuticals from plants. However, several bottlenecks including regulatory guidelines, ethical issues and public approval must be taken into account and solved [1]. References: Hashemzadeh, H. and Zebarjadi, A. (2014). Application of transgenic plants as factories for producing biopharmaceutical. [online] www.researchgate.net. Available at: https://www.researchgate.net/publication/268514150_Application_of_transgenic_plants_as_factories_for_producing_biopharmaceutical?enrichId=rgreq-4465fb2b3b0479dec4752b8195daab6c-XXXenrichSource=Y292ZXJQYWdlOzI2ODUxNDE1MDtBUzoxNjU2NDA4MDM0NjMxNjhAMTQxNjUwMzI1MzMxOA%3D%3Del=1_x_3_esc=publicationCoverPdf [Accessed 17 Mar. 2017]. Frenzel, A., Hust, M. and Schirrmann, T. (2013). Expression of Recombinant Antibodies. [online] journal.frontiersin.org. Available at: http://journal.frontiersin.org/article/10.3389/fimmu.2013.00217/full [Accessed 17 Mar. 2017]. Moussavou, G., Ko, K., Lee, J. and Choo, Y. (2015). Production of Monoclonal Antibodies in Plants for Cancer Immunotherapy. [online] Hindawi.com. Available at: https://www.hindawi.com/journals/bmri/2015/306164/ [Accessed 17 Mar. 2017]. Valdà ©s, R., Padilla, S., Gà ³mez, L. and Borroto, C. (2003). Large-scale purification of an antibody directed against hepatitis B surface antigen from transgenic tobacco plants. [online] www.researchgate.net. Available at: https://www.researchgate.net/publication/10635451_Large-scale_purification_of_an_antibody_directed_against_hepatitis_B_surface_antigen_from_transgenic_tobacco_plants?enrichId=rgreq-9f9d9c1d7e1ac5013b459ed8c8d81938-XXXenrichSource=Y292ZXJQYWdlOzEwNjM1NDUxO0FTOjk4OTYxOTIyNzIzODQ1QDE0MDA2MDU3NjkyNjc%3Del=1_x_3_esc=publicationCoverPdf [Accessed 17 Mar. 2017]. Pujol, M. and Gavilondo, J. (2013). Transgenic plants of Nicotiana tabacum L. express aglycosylated monoclonal antibody with antitumor activity. [online] www.researchgate.net. Available at: https://www.researchgate.net/publication/263802548_Transgenic_plants_of_Nicotiana_tabacum_L_express_aglycosylated_monoclonal_antibody_with_antitumor_activity?enrichId=rgreq-e820e9544359d3a71edb5ce55845f43a-XXXenrichSource=Y292ZXJQYWdlOzI2MzgwMjU0ODtBUzoxMTczNzkwNTI4NzE2ODFAMTQwNDk5Njc1NTQwMg%3D%3Del=1_x_3_esc=publicationCoverPdf [Accessed 17 Mar. 2017]. Hayes C. Generation and Application of Recombinant Antibody Fragments for Prostate Cancer Detection. A thesis submitted for the degree of Ph.D. School of Biotechnology, Dublin City University,Dublin 9,Ireland 2010 j6) Rodrà ­guez, M., Pà ©rez, L., Gavilondo, J., Garrido, G., Bequet-Romero, M., Hernà ¡ndez, I., Huerta, V., Cabrera, G., Pà ©rez, M., Ramos, O., Leyva, R., Leà ³n, M., Ramos, P., Triguero, A., Hernà ¡ndez, A., Sà ¡nchez, B., Ayala, M., Soto, J., Gonzà ¡lez, E., Mendoza, O., Tiel, K. and Pujol, M. (2012). Comparative in vitro and experimental in vivo studies of the anti-epidermal growth factor receptor antibody nimotuzumab and its aglycosylated form produced in transgenic tobacco plants. [online] http://onlinelibrary.wiley.com. Available at: http://onlinelibrary.wiley.com/doi/10.1111/pbi.12006/full [Accessed 17 Mar. 2017]. Yu-Cai Liao , He Pingli , Chun Senzhao , Ming-Jing Yao , Jing-à ¢Ã¢â€š ¬Ã‚ ¨Bo Zhang Jin-Long Liu (2006) Plantibodies: A Novel Strategy to Create Pathogen- Resistant Plants, Biotechnology and Genetic Engineering Reviews, 23:1, 253-272, DOI: 10.1080/02648725.2006.10648087. Xu, J., Ge, X. and C. Dolan, M. (2011). Towards high-yield production of pharmaceutical proteins with plant cell suspension cultures. [online] Sciencedirect.com. Available at: http://www.sciencedirect.com/science/article/pii/S0734975011000036 [Accessed 17 Mar. 2017]. Innovateus.net. (n.d.). What is DNA Particle Bombardment?. [online] Available at: http://www.innovateus.net/science/what-dna-particle-bombardment [Accessed 17 Mar. 2017]. Clark, D. and Pazdernik, N. (2009). Biotechnology. 1st ed. Beijing: Ke xue chu ban she, p.405. 1.Adams GP, Weiner LM. Monoclonal antibody therapy of cancer. Nat Biotechnol 2005 Sep;23(9):1147-57. 2.Souriau C, Hudson PJ. Recombinant antibodies for cancer diagnosis and therapy. Expert Opin on Biolog Ther 2003 Apr;3(2):305-18. Hayes C. Generation and Application of Recombinant Antibody Fragments for Prostate Cancer Detection. A thesis submitted for the degree of Ph.D. School of Biotechnology, Dublin City University, Dublin 9, Ireland 2010. Gà ³mez, L., Valdà ©s, R., Pujol, M. and Brito, J. (2011). Kinetic of Expression of a Plantibody in (Nicotiana tabacum) Plants Cultivated in Different Substrates (Zeolite and Soil). [online] www.researchgate.net. Available at: https://www.researchgate.net/publication/287551483_Kinetic_of_Expression_of_a_Plantibody_in_Nicotiana_tabacum_Plants_Cultivated_in_Different_Substrates_Zeolite_and_Soil [Accessed 17 Mar. 2017]. Vasilev, N. (2015). Developments in the production of mucosal antibodies in plants. [online] Sciencedirect.com. Available at: http://www.sciencedirect.com/science/article/pii/S0734975015300525 [Accessed 17 Mar. 2017].

Natural Law Theory Essay -- essays research papers fc

Natural Law Theory The natural law theory is a theory that dates back to the time of the Greeks and great thinkers like Plato and Aristotle. Defined as the law which states that human are inborn with certain laws preordained into them which let them determine what is right and what is wrong.(Bainton 174) This theory was them adapted by religious philosophers to fit the Christian religion.(Berkhof 114) This, however was not exactly the same as the original. The classical thinkers were the first to define the natural law. Heraclitus, in the sixth century BC, specified one the components by saying, *for all human laws are nourished by one, the divine.* This meant that a divine power determined a logic and gave to all humans. (Microsoft Encarta) This definition put this law into direct conflict with positive laws. Aristotle elaborated on the word natural in relation to law. He said that a natural law was one that had the same validity for every one and situation.(Berkhof 268) An example of this would be that a man contemplating murder would see that it was wrong by his nature. His reason would tell him that to kill another was unnatural, and therefore wrong. Cicero tries to determine what the actual law encompassed and he came up with the theory of Stoicism. Stoicism is an interpretation of the natural law which states that every, single person is a part of the universe that was created and is ruled by a divine power rationally. To live rationally and with virtue, according to...

Hucks Contradiction in The Adventures of Huckleberry Finn Essay

Huck's Contradiction in The Adventures of Huckleberry Finn      Ã‚  Ã‚   In Mark Twain's The Adventures of Huckleberry Finn Huck was a boy who thought very little of himself, but had a huge impact on others.   His moral standing was based on what is easier, right or wrong.   He lived the way he wanted to live, and no one told him otherwise.   He had the adventure of a lifetime, and yet he learned along the way. Although Huck has certain beliefs about himself, his actions and decisions contradict these beliefs.      Ã‚  Ã‚  Ã‚  Ã‚   Huck may consider himself lazy, but in reality, he is a very hard worker.   At one point, Huck wants to get away from his father so he comes up with a scheme to fake his death and escape from his cabin:   "I out with my saw and went to work on that log again. I took the sack of corn meal and took it to where the canoe was hid and shoved the vines and branches apart and put it in. I had wore the ground a good deal, crawling out of the hole and dragging out so many things.   So I fixed that as good as I could from the outside. Then I fixed the piece of log back into its place. I took the ax and smashed in the door-I beat it and hacked it considerable, a-doing it.   I fetched the pig.and laid him down on the ground to bleed. Well, last I pulled out some of my hair, and bloodied the ax good, and stuck it on the back side, and slung the ax in the corner" (24).   If Huck were lazy, he would not have gone through all that trouble to escape, if he escaped at all.   A lazy person would have just stayed there and not worried about what happened.   At another point in the novel, Huck and a runaway slave, Jim, are on an island where th... ...x, James M. From Mark Twain: The Fate of Humor (Princeton University Press, 1966) "Southwestern Vernacular" pp. 167-184. Copyright @1966 by Princeton University Press. Rpt. Twentieth Century Interpretations of Adventures of Huckleberry Finn Ed. Claude M Simpson. Englewood Cliffs,N.J. 1968.    Fishkin, Shelley Fisher, Phd. "Teaching Mark Twain's Adventures of Huckleberry  Ã‚  Ã‚  Ã‚   Finn", 1995, July Summer Teachers Institute, Hartford, Connecticut @1995    http://www.pbs.org/wgbn/cultureshorck/teachers/huck/essay.html    Leavis, F.R. "Three New Approaches to Huckleberry Finn". (London: Chatto and   Windus, Ltd., 1955) Rpt. Twentieth Century Interpretations of Adventures of  Ã‚   Huckleberry Finn Ed. Claude M Simpson. Englewood Cliffs,N.J. 1968.    Twain, Mark. The Adventures of Huckleberry Finn. Berkeley: University of California Press, 2001.   

Who do you think is more successful leader Ralph or Jack? :: Free Essay Writer

Who do you think is more successful leader Ralph or Jack? Ralph'sElected the leader of the boys at the beginning of the novel, Ralph is the primary representative of order, civilization, and productive leadership in the novel. While most of the other boys are concerned with playing, having fun, and avoiding work at the beginning of the novel, Ralph sets about building huts and thinking of ways to maximize their chances of being rescued. For this reason, Ralph's power and influence over the other boys are extremely secure at the beginning of the novel. However, as the book progresses and the group succumbs to savage instincts, Ralph's position declines precipitously as Jack's station rises. Eventually, all the boys leave Ralph's group for Jack's, and Ralph is left alone to be hunted by Jack's tribe. Ralph never seriously considers joining Jack's tribe in order to save himself. Ralph's commitment to civilization and morality is very strong, and his main wish is to be rescued and returned to the society of adults. In a sense, this strength gives Ralph a moral victory at the end of the nova, leadership qualities were to inspire confidence by providing hope for the rest of the group (we will get food hunt. Catch things until they fetch us), or example; the fire was used to signal and to attract only attentions he said (you rub two sticks. you rub), to any passing ships or planes. We see many events through Ralph's eyes, and are given many insights into the way he feels about events and people. Ralph was liked thus he was chosen as leader, he was fair, by him giving the group the right to speak whenever they are holding the conch. As always a successful leader needs some courage to face enemies and others who try to intrude, against his followers with his superiority of mind control and not physically has a set of rules; and by definition of LEADER is a person who rules or guides or inspires others. Ralph's character does not only lead the group, but he also attracts us the readers too, to the novel and also makes us, the readers want to know what would happen and if he is going to be successful doing his job as a leader or not. When the little ones do not help out Ralph does not react in a violent way he is calm and relaxed about them building sand castles, because he does not expect them to help. yet because they have had less time learning about the ways of society. By the end of the book, Ralph's position changes and shows that his

Apush Notes

1. The Shaping of North America 1. Recorded history began 6,000 years ago. It was 500 years ago that Europeans set foot on the Americas to begin the era of accurately recorded history on the continent. 2. The theory of â€Å"Pangaea† exists suggesting that the continents were once nestled together into one mega-continent. The continents then spread out as drifting islands. 3. Geologic forces of continental plates created the Appalachian and Rocky Mountains. 4. The Great Ice Age thrust down over North America and scoured the present day American Midwest. 2. Peopling the Americas 1.The â€Å"Land Bridge† theory†¦ 1. As the Great Ice Age diminished, so did the glaciers over North America. 2. The theory holds that a â€Å"Land Bridge† emerged linking Asia & North America across what’s today the Bering Sea. People were said to have walked across the â€Å"bridge† before the sea level rose and sealed it off and thus populated the Americas. 2. The Lan d Bridge is suggested as occurring an estimated 35,000 years ago. 3. Many peoples emerged†¦ 1. Those groups that traversed the land bridge spread across North, Central, and South America. 2. Countless tribes emerged with an estimated 2,000 languages.Notably†¦ 1. Incas – Peru, with elaborate network of roads and bridges linking their empire. 2. Mayas – Yucatan Peninsula, with their step pyramids. 3. Aztecs – Mexico, with step pyramids and huge sacrifices of conquered peoples. 3. The Earliest Americans 1. Development of corn or â€Å"maize† around 5,000 B. C. in Mexico was revolutionary in that†¦ 1. Then, people didn’t have to be hunter-gatherers, they could settle down and be farmers. 2. This fact gave rise to towns and then cities. 3. Corn arrived in the present day U. S. around 1,200 B. C. 2. Pueblo Indians 1.The Pueblos were the 1st American corn growers. 2. They lived in adobe houses (dried mud) and pueblos (â€Å"villages† in Spanish). Pueblos are villages of cubicle shaped adobe houses, stacked one on top the other and often beneath cliffs. 3. They had elaborate irrigation systems to draw water away from rivers to grown corn. 3. Mound Builders 1. These people built huge ceremonial and burial mounds and were located in the Ohio Valley. 2. Cahokia, near East St. Louis today, held 40,000 people. 4. Eastern Indians 1. Eastern Indians grew corn, beans, and squash in â€Å"three sister† farming†¦ 1.Corn grew in a stalk providing a trellis for beans, beans grew up the stalk, squash’s broad leaves kept the sun off the ground and thus kept the moisture in the soil. 2. This group likely had the best (most diverse) diet of all North American Indians and is typified by the Cherokee, Creek, Choctaw (South) and Iroquois (North). 5. Iroquois Confederation 1. Hiawatha was the legendary leader of the group. 2. The Iroquois Confederation was a group of 5 tribes in New York state. 3. They were matri lineal as authority and possessions passed down through the female line. 4.Each tribe kept their independence, but met occasionally to discuss matters of common interest, like war/defense. 5. This was not the norm. Usually, Indians were scattered and separated (and thus weak). 6. Native Americans had a very different view of things as compared to Europeans. 1. Native Americans felt no man owned the land, the tribe died. (Europeans liked private property) 2. Indians felt nature was mixed with many spirits. (Europeans were Christian and monotheistic) 3. Indians felt nature was sacred. (Europeans believed nature and land was given to man by God in Genesis to be subdued and put to use). . Indians had little or no concept or interest in money. (Europeans loved money or gold) 4. Indirect Discoverers of the New World1. The 1st Europeans to come to America were the Norse (Vikings from Norway). 1. Around 1,000 A. D. , the Vikings landed, led by Erik the Red and Leif Erikson. 2. They landed i n â€Å"Newfoundland† or â€Å"Vinland† (due to all of the vines). 3. However, these men left America and left no written record and therefore didn’t get the credit. 4. The only record is found in Viking sagas or songs. 2. The Christian Crusaders of Middle Ages fought in Palestine to regain the Holy Land from Muslims.This mixing of East and West created a sweet-tooth where Europeans wanted the spices of the exotic East. 5. Europeans Enter Africa This content copyright  © 2010 by WikiNotes. wikidot. com 1. Marco Polo traveled to China and stirred up a storm of European interest. 2. Mixed with desire for spices, an East to West (Asia to Europe) trade flourished but had to be overland, at least in part. This initiated new exploration down around Africa in hopes of an easier (all water) route.3. Portugal literally started a sailing school to find better ways to get to the â€Å"Spice Islands,† eventually rounding Africa’s southern Cape of Good Hope. . New developments emerged†¦ 1. caravel – a ship with triangular sail that could better tack (zig-zag) ahead into the wind and thus return to Europe from the Africa coast. 2. compass – to determine direction. 3. astrolabe – a sextant gizmo that could tell a ship’s latitude. 5. Slave trade begins 1. Slavery was initially race-independent. A slave was whoever lost in battle. Usually, slaves came from the Slavic regions of Europe, hence the name. 2. The first African slave trade was across the Sahara Desert. 3. Later, it was along the West African coast.Slave traders purposely busted up tribes and families in order to squelch any possible uprising. 4. Slaves wound up on sugar plantations the Portuguese had set up on the tropical islands off of Africa’s coast. 5. Spain watched Portugal’s success with exploration and slaving with envy and wanted a piece of the pie. 6. Columbus Comes upon a New World 1. Columbus convinced Isabella and Ferdina nd to fund his expedition. 2. His goal was to reach the East (East Indies) by sailing west, thus bypassing the around-Africa route that Portugal monopolized. 3.He misjudged the size of the Earth though, thinking it 1/3 the size of what it was. 4. So, after 30 days or so at sea, when he struck land, he assumed he’d made it to the East Indies and therefore mistook the people as â€Å"Indians. † 5. This spawned the following system†¦ 1. Europe would provide the market, capital, technology. 2. Africa would provide the labor. 3. The New World would provide the raw materials of gold, soil, and lumber.7. When Worlds Collide 1. Of huge importance was the biological flip-flop of Old and New Worlds. Simply put, it was a trade of life such as plants, foods, animals, germs. . From the New World (America) to the Old 1. corn, potatoes, tobacco, beans, peppers, manioc, pumpkin, squash, tomato, wild rice, etc. 2. also, syphilis 3. From Old World to the New 1. cows, pigs, horses, wheat, sugar cane, apples, cabbage, citrus, carrots, Kentucky bluegrass, etc. 2. devastating diseases – smallpox, yellow fever, malaria as Indians had no immunities. 1. The Indians had no immunities in their systems built up over generations. 2. An estimated 90% of all pre-Columbus Indians died, mostly due to disease. 8. The Spanish Conquistadores 1.Treaty of Tordesillas, 1494 – Portugal and Spain feuded over who got what land. The Pope drew this line as he was respected by both. 1. The line ran North-South, and chopped off the Brazilian coast of South America 2. Portugal got everything east of the line (Brazil and land around/under Africa) 3. Spain got everything west of the line (which turned out to be much more, though they didn’t know it at the time) 2. Conquistadores is Spanish â€Å"conquerors†. 1. Vasco Balboa – â€Å"discovered† the Pacific Ocean across the isthmus of Panama.2. Ferdinand Magellan – circumnavigated the globe (h e was the first to do so). . Ponce de Leon – touches and names Florida looking for legendary â€Å"Fountain of Youth†. 4. Hernando Cortes – enters Florida, travels up into present day Southeastern U. S. , dies and is â€Å"buried† in Mississippi River, 5. Francisco Pizarro – conquers Incan Empire of Peru and begins shipping tons of gold/silver back to Spain. This huge influx of precious metals made European prices skyrocket (inflation). 6. Francisco Coronado – ventured into current Southwest U. S. looking for legendary Cibola, city of gold. He found the Pueblo Indians. 3. Encomienda system established 1.Indians were â€Å"commended† or given to Spanish landlords 2. The idealistic theory of the encomienda was that Indians would work on the farm and be converted to Christianity. But it was basically just slavery on a sugar plantation guised as missionary work. 9. The Conquest of Mexico 1. Hernando Cortez conquered the Aztecs at Tenochti tlan. 2. Cortez went from Cuba to present day Vera Cruz, then marched over mountains to the Aztec capital. 3. Montezuma, the Aztec king, thought Cortez might be the god Quetzalcoatl who was due to re-appear that very year. Montezuma welcomed Cortez into Tenochtitlan. . The Spanish lust for gold led Montezuma to attack on the noche triste, sad night. Cortez and men fought their way out, but it was smallpox that eventually beat the Indians. 5. The Spanish then destroyed Tenochtitlan, building the Spanish capital (Mexico City) exactly on top of the Aztec city. 6. A new race of people emerged, mestizos, a mix of Spanish and Indian blood. 10. The Spread of Spanish America 1. Spanish society quickly spread through Peru and Mexico 2. A threat came from neighbors†¦ 1. English – John Cabot (an Italian who sailed for England) touched the coast of the current U.S. 2. Italy – Giovanni de Verrazano also touched on the North American seaboard. 3. France – Jacques Cartier went into mouth of St. Lawrence River (Canada). 3. To oppose this, Spain set up forts (presidios) all over the California coast. Also cities, like St. Augustine in Florid # 4. Don Juan de Onate followed Coronado’s old path into present day New Mexico. He conquered the Indians ruthlessly, maiming them by cutting off one foot of survivors just so they’d remember. 5. Despite mission efforts, the Pueblo Indians revolted in Pope’s Rebellion. 6.Robert de LaSalle sailed down the Mississippi River for France claiming the whole region for their King Louis and naming the area â€Å"Louisiana† after his king. This started a slew of place-names for that area, from LaSalle, Illinois to â€Å"Louisville† and then on down to New Orleans (the American counter of Joan of Arc’s famous victory at Orleans). 7. â€Å"Black Legend† – The Black Legend was the notion that Spaniards only brought bad things (murder, disease, slavery); though true, they also brought good things such as law systems, architecture, Christianity, language, and civilization, so that the Black Legend is partly, but not entirely, accurate.

Breaking Through Essay

Throughout the book â€Å"Breaking Through,† Francisco Jinemez discusses many themes. This book is an interesting and a must read book for people who moving to another country and trying to get a better life. There are three themes in the novel that one must follow in order to understand and earn a meaningful life: family, work, and education. First of all, family is the first school in which a child receives the basic values of life. When Francisco was young, his family moved to America, hoping to get a better life. He grew up in a poor family. The six members in his family are his parents, (how many? 3? ) brothers, and sister. His family suffers and struggles with many hardships because they were not coherent English speakers. His mother is a caring and thoughtful woman who places her family as an important priority. She gives Francisco a lot of advice that helped him solve many of his problems. All the members of his family are friendly except his father. His father always yells at him and Roberto when they ask to go out. Although his mood changes invariably like the weather, they still respect him completely. Francisco spends a lot of time with his big brother since their ages are close to each other. Francisco’s love for his family is never-ending. In one part of the book, Francisco and his brother, Roberto, moved away from their family to a different city to live by themselves. At the beginning, Francisco misses his family so much that he can’t help but reminisce about the time when they still lived together. Francisco often misses â€Å"the sounds of [his] Papa’s coughing, the rattle of his aspirin bottle, and the rolling of Mama’s twelve-inch lead pipe† (19). Being away from his family occasionally makes Francisco think of all the little things about his life when he lived with his parents. Jimeneez illustrated an image of Francisco’s life before he moved to _____ in order to demonstrate how Francisco’s strong ties to his family causes him to think about when they were still living together. Everyone has to work whether to earn money or get experience for a better future. When Francisco was still in middle school, he supported his family by working. His work was to pick cotton, carrot, or strawberry, depending on the season. Francisco’s father has trouble with his back, so he could not work as well as he did before. Things get worse when Roberto got married because he is unable to support the family anymore because he needs the money to build his own family. Francisco and his father become depressed as they worry for their life and survival. They often wonder how they will survive with the limited amount of money they receive from their work. Life is tough for them. Why do we have to go to school? It is the best option for our future. In general, achieving an education is not an easy thing to do. It is even harder for Francisco who moved to another country. Although he has problem with English, he never stop trying to learn it. In addition to getting a good education, Francisco also has to help support his family. This caused him to miss many school days. In his senior year, he hears good news from his teacher that he might be able to get a scholarship. He discusses the news with his family, but his father did not approve. Francisco could not control himself and finally talk back to his father that why cannot he get into college. Francisco felt ‘’anger [swallowing him] and [he] could not escape it† (168) Francisco felt passionate about his education because it is the only hope for Francisco to escape his poor life. He believes that he must fight for it because education is not only a ladder to success, but it will also give him knowledge to become a useful person in the future. In conclusion, Jimenez is trying to demonstrate survival and hardship through these themes. These are themes that would help us break through all the hardships in life. A better future depends on our hope and courage.

Checkpoint: Evaluating Communication Strategies

Resources: pp. 195–210, 214–220, & 223–226 in Ch. 5 of Human Services in Contemporary America Answer the following questions in 200 to 300 words:†¢ What would you need to learn or know about each of your clients in order to communicate effectively with them? The text explains that culture/religion should be on a human service workers list of things to know. There are many different types of cultures and beliefs that go along with those cultures. For instance, there are cultures that believe it is ok to abuse the woman and while as human service workers, we do not agree, that is their culture and it is our job to help them see that it is not acceptable in the United States and there are consequences for violence against any human being especially domestic violence. I also believe that a human service worker should know what kind of lifestyle the person was raised in or where they grew up (i.e. the projects, ghetto, wealthy area, a different country etc).†¢ What strategies or techniques do you believe would be the best approach to take when communicating with each of these clients? I believe that being compassionate, empathetic, and genuineness is good approaches to communicating with clients. I also feel that being able to communicate well and listening are also good approaches to clients.†¢ How might you need to adapt your communication approach from one individual to the next? Every single person is different which means there are different personalities, cultures, religions and beliefs. While we may not always agree with the person we are trying to help, we need to be able to put ourselves in their shoes or at least know something about the person so that we can decide what the best way to help the person is.There are also different situations in which the worker should be able to adapt to. For example, one day I may work with a child who has serious mental health issues in which they may have a serious personality disorder and the next day I may work with a rape victim and my approach will change from case to case.

Greatest Place on Earth Essay

I believe the Greatest Place on Earth is Greenville Tennessee. The time that me and my family spend there are magical. I call Greenville Tennessee the Greatest Place on Earth because of the times and the memories that has been spent and made there. The country roads are so peaceful and clean, the air is fresh and the people are super nice. There are so many things there to do and most of the things that we have found are free. There is the beautiful waterfall that is so peaceful to take the kids to play while you sit by and read a book and drift away into what I call the fantasy world. The kids will play for hours in the falls a beautiful place where my family and I love to take pictures and capture the moments of our lives. Where the waterfall has crystal clear water and the sounds from it is so peaceful and relaxing my family always chose to go to the waterfall for those very reasons. There is also a place where there is small sandy beach better known as the kids own mini personal beach where they enjoy the hot summer days of swimming, building sand castles, and playing beach volleyball with the rest of the family. The family sits back and lets the children enjoy the sun and sand while they relax in the sun with a book or listen to music as memories are made once again. The children all feel special because the small sandy beach is what they like to call their very own. Then there is what we all call outlook landing it’s an intense hike up a mountain side in the woods that will leave to tired and sweating, but well worth the hike. Once getting to the top all the stress that the world throws your way leaves and the sight is the most beautiful thing that my eyes have ever saw. The mountain is such a beautiful sight being able to see for miles and miles around, with the big rocks to set on and look out over the mountain you are able to see the three surrounding states. Packing drinking supplies and food to cook while on the mountain we set under the shelter and enjoy the view as the sun is about to set . Every season is different the fall the leaves are starting to turn beautiful shades or orange, yellow, and red the climate is starting to change as the days are getting cooler. When in the summer months the sun is bright, the birds chirp, the sound of traffic is never heard, the cars and trucks on the tiny road ways look like ants. Spring time the flowers are blooming, leaves are growing back, animals are starting to come alive, the weather is starting to warm and view is amazing while all the beautiful things are growing. Outlook Mountain is one of the places where my family and I enjoy spending time while visiting the greatest place on earth. While staying in Greenville Tennessee my family and I also spend time at a private family owned fishing lake. When spending a day at the lake the whole family gets together and sits back to fish and talks about the memories make at the lake. Nothing is better than making a fire and letting the kids chose their own sticks to make their roasted hotdogs and marshmallows for their homemade smores. The children are having the best day catching fish and taking pictures so that they are able to show how well they did on their fishing trip that day. With a fun filled day the children have dirty hands and faces to prove their great time at the lake. As the day slowly starts to end the family starts to pack up all the fishing gear to rest up for another fun filled day in the morning. Greenville Tennessee is the Greatest Place on Earth because there are so many things that we as a family love to do. When our family takes a trip to the Greatest Place on Earth we never know what the next day will have in store for us. Maybe it’s taking a trip to Dollywood, Cherokee, a day of shopping the waterfall, outlook mountain, or the fishing lake my family and I always knows that when heading to Greenville Every trip that my family and I make to Tennessee is always filled with fun and memories are always make. As our trip is near the end, we look forward to the next time that we get to visit Greenville Tennessee for more family fun. That is why Greenville Tennessee is the Greatest Place on Earth.

Phase3 DB International Business Disputes Essay Example | Topics and Well Written Essays - 500 words

Phase3 DB International Business Disputes - Essay Example This assignment provides a compressive discussion on the consequences that a business would face for operating within states that have not agreed with the United Nations Convention on Contracts for the International Sale of Goods. It also gives detailed information on whether a business should or should not engage in business with nations that have not conformed to the requirements of the United Nations Convention on Contracts for the International Sale of Goods. One of the major consequences for transacting business with states that have not conformed to the United Nations Convention on Contracts for the International Sale of Goods is that only the contract laws of such nations will apply (Ferrari, 2012). This implies that an enterprise that wishes to engage in such business in capable to inadequate treatments in accordance with the laws of the state that has not agreed with the terms of the CISG. The contract law of such a nation may not be accommodative to foreign businesses. It is for this reason that such nations may be weaker in ensuring that the contractual balance between foreign and indigenous businesses is maintained. Businesses that wish to operate within states that have not agreed with the terms and conditions of the CISG may have to face importation or exportation restrictions according to the governing laws. It is important to note that different nations have different laws governing the importation and exportation of certain goods. However, the CISG standardizes the importation and exportation of goods across borders. Therefore, a business that wishes to transact business with states that have failed to embrace this international law may have to face the restriction of the importation or exportation of certain goods. According to article 68 of the CISG, the risk of damage of goods is passed to the buyer once the goods have been delivered. However, an enterprise engaging in business

Applied Research Methods Essay Example | Topics and Well Written Essays - 3000 words

Applied Research Methods - Essay Example This in turn should lead to the development of more efficient and effective business processes and organizational decision making systems. This entire system will enhance an organization’s ability to sense and respond to changes in its external environment, which will increase its chances of survival in today hostile business environment. Critical Literature Review: Knowledge Management Knowledge management is the vehicle by which organization enhance and encourage organizational learning. Knowledge management is a vehicle by which an organization can enhance its ability to learn from its environment and to incorporate knowledge into its business processes (McAdam and Reid, 2001). There are four value-adding steps in the knowledge management value chain, these are as follows (Wong and Aspinwall, 2005): Knowledge Acquisition: Organizations can acquire knowledge in multiple ways, this depends on the type of knowledge they seek to acquire. Industry libraries comprise of industry related documents, reports, presentations, and best practices. Moreover, employees of the industry are encouraged to add new knowledge based on their experiences. An industry can acquire new knowledge by developing online expert networks; this kind of network facilitates an employee to seek advice from an expert in the company who has the knowledge of the problem area in his or her head. A company operating in a particular industry can create new knowledge by discovering patterns in corporate data or by using knowledge workstations. Knowledge Storage: after discovering the fundamental patterns, documents and experts rules there need to be a repository of this industry. This data should be accessible to any new entrant or to any already operating in the industry. Knowledge Dissemination: Portals, e-mail, instant messaging and searching technology can bring about the dissemination of desired knowledge. These technologies in addition to an array of groupware technologies can increase t he rate of dissemination of information. Knowledge Application: Gathered knowledge that does not get shared and applied to a practical problem faced by a firm operating in a particular industry, than there would no value added in the current business practices of the industry. Thus in this scenario the only plausible outcome of this lackluster attitude of the firms of a particular industry is stagnation and in turn eradication from the industry. Role of Small and medium enterprises in Developing Economies One important aspect of a flourishing economy is its nourishing and germinating small and medium enterprise. The fundamental points that differentiate an SMALL AND MEDIUM ENTERPRISE from large scale enterprises are uncertainty, innovation and evolution. This sector of SMALL AND MEDIUM ENTERPRISE is further classified as micro enterprises, small enterprises, and medium enterprises. These small enterprises create the most fertile ground for the germination of industrial economy. Sinc e this industry plays a vital role in the following economic paradigms therefore it is of utmost importance that a country generates and gathers all the pertinent information that will allow the entrepreneurs of the company to nurture this sector: income distribution, tax revenue, employment, resource utilization, development of family life and etch. By having a major